Journal: PLoS ONE

Article Title: Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice

doi: 10.1371/journal.pone.0147173

Figure Lengend Snippet: Cells were grown and immunostained with CAR, integrin α v β 3 , or α v β 5 antibody and then subjected to flow cytometric analysis. Columns, percentages of cells expressing CAR, αvβ3 and αvβ5. Data are presented as means ± standard deviation for three independent experiments.

Article Snippet: To detect expression of CAR and integrin α v β 3 and α v β 5 , the cells were detached using an enzyme-free dissociation solution and then incubated with a mouse monoclonal anti-human CAR antibody (Upstate Biotechnology, Lake Placid, NY, USA), a mouse monoclonal anti-human integrin α v β 3 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA), or a mouse monoclonal anti-human integrin α v β 5 antibody (R&D Systems, Inc., Minneapolis, MN, USA), all at a dilution of 1:100 for 1 h on ice.

Techniques: Expressing, Standard Deviation